Journal: PLoS ONE

Article Title: Inhibition of Glioma Cell Lysosome Exocytosis Inhibits Glioma Invasion

doi: 10.1371/journal.pone.0045910

Figure Lengend Snippet: GPN treatment decreased glioma cell surface lysosome number. A. Upper: representative confocal images depicting the astrocyte surface Lamp1 (green) and the total Lamp1 (red). Lower: quantification of the intensity ratio (surface/total). Error bars represent the s.e.m. of 42 images from two independent experiments; ***denotes P<0.001 of C6 compared with astrocytes. (Student's t-test). B. The FACS results of LAMP1 surface staining showing more lysosomes on the surface in both C6 and U251 glioma cells than in astrocytes. GPN treatment decreased LAMP1 surface staining compared with the control group. The X axis shows fluorescence intensity and the Y axis shows cell number. The green curve represents the negative control and the red area represents the LAMP1 surface staining group.

Article Snippet: One group was incubated with 50 μl mouse anti-LAMP1 antibody (Assay Designs, VAM-EN001, 1∶50) at 4°C for 1 h, and the other was incubated with the same amount of PBS.

Techniques: Staining, Control, Fluorescence, Negative Control

Journal: PLoS ONE

Article Title: Inhibition of Glioma Cell Lysosome Exocytosis Inhibits Glioma Invasion

doi: 10.1371/journal.pone.0045910

Figure Lengend Snippet: C6 and U251 cells were immunostained with LAMP1 (green), cathepsin D (red), and Rab27a (blue). Bar charts show the percentage of colocalized puncta (labeled by both types of fluorescent dye). The number associated with each column refers to the number of image fields examined for each condition.

Article Snippet: One group was incubated with 50 μl mouse anti-LAMP1 antibody (Assay Designs, VAM-EN001, 1∶50) at 4°C for 1 h, and the other was incubated with the same amount of PBS.

Techniques: Labeling

Journal: Renal Failure

Article Title: Targeting LRH-1 alleviates diabetes-induced lipotoxicity in podocytes: role of PLIN5-mediated lipid droplet turnover

doi: 10.1080/0886022X.2026.2670806

Figure Lengend Snippet: LRH-1 overexpression alleviated HG-induced lipid accumulation and lipid droplet turnover. (A) ORO staining of cultured podocytes. HPCs were treated with HG for 24 hours. (B–D) Western blot assay showing expression of LRH-1 and PLIN5 among different treatment groups and quantitative analyses ( n = 6). (E) The relative MDA among different groups ( n = 6). (F) Quantitative analysis of podocytes FFA in each group ( n = 3). (G) Quantitative analysis of podocytes ROS levels in each group ( n = 6). (H) Representative images and quantification of MitoTracker (red), BODIPY (green), and DAPI (blue)-immunofluorescent stained podocytes from each group ( n = 6). (I) Representative images and quantification of LAMP1 (red), BODIPY (green), and DAPI (blue)-immunofluorescent stained podocytes from each group ( n = 6). * p < 0.05 and ** p < 0.01. Five visual fields for each section analyzed.

Article Snippet: Rabbit anti-LRH-1 antibodies and rabbit anti-PLIN5 antibodies were obtained from Proteintech (Wuhan, China); mouse anti-Wilms’ tumor 1 (WT1) antibodies were purchased from Novus (Centennial, CO); mouse anti-Lamp1 antibodies were purchased from Progen Biotechnik (Heidelberg, Germany); anti-β-actin mouse monoclonal antibody, fluorescent secondary antibodies were purchased from Antgene (Wuhan, China).

Techniques: Over Expression, Staining, Cell Culture, Western Blot, Expressing